Thymoquinone Induces Human Platelet Aggregation via Cytochrome P450 and Mitochondria

Platelets are small circulating anucleated cells, resulting from the fragmentation of megakaryocytes. They directly involved in primary hemostasis, where they among first elements to intervene stopping bleeding. Platelet count or functional alteration was reported be correlated different types diseases. Since antiquity, plants have been used cure panoply Nigella sativa, black cumin, is an annual Mediterranean plant. sativa's main essential oil constituent, thymoquinone (30 %-48 %) responsible for a number its biological activity. Various drugs and plant substances can induce thrombocytopenia. Some studies describe dose time-dependent decrease platelet induced by consumption these substances. Thymoquinone quinine-like compound; quinines drug-induced immune Indeed, human rat administration seed 4 12 w, respectively, To date, effect on platelets not fully understood. Here we bring new effects aggregation. Our results show that itself able aggregation, but needs relatively long exposure (28 min). We also thymoquinone-induced aggregation probably mediated through cytochrome metabolites mainly via mitochondrial pathway. Results demonstrate pre-incubation with abrogate thrombin-induced concentration-dependent manner. In conclusion, seeds as nutritional complement alternative medicine should low concentration under phyto-specialists medical supervision.